General Cell Based Assays
Meso Scale Discovery R-PLEX Rat IL-5 ASY
The R-PLEX Rat IL-5 Assay Kit from Meso Scale Discovery is an electrochemiluminescent immunoassay for the quantitative measurement of IL-5 in rat serum plasma and cell culture supernatants The assay uses MSD GOLD SULFO-TAG labels and has a lower limit of detection of 1 15 pg/mL with an upper limit of quantification of 1500 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator diluent and a vial of lyophilized calibrator The assay takes approximately 3 5 hours to run
Revvity Health Sciences Inc HTRF Human Total ZAP70 Detection Kit, 10,000 Assay Points
HTRF Human Total ZAP70 Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter Human ADCYAP1R1 (PAC1) Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human ADCYAP1R1 (PAC1) Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure ADCYAP1R1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Revvity Health Sciences Inc HTRF α-Tubulin Housekeeping Kit, 50,000 Assay Points
HTRF α-Tubulin Housekeeping Kit, 50,000 Assay Points
Enzo Life Sciences Cathepsin K drug discovery kit (1x96wells)
The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit. Applications: Fluorescent detection, HTS Activity assay.
Cayman Chemical LPO asy TrIphenylphosphIn 1ea
A menin-MLL1 protein-protein interaction inhibitor binds to menin (Kd 22 nM) and inhibits the interaction between menin and MLL1 in a cell-free assay (IC50 46 nM) reduces cell growth (GI50 3 UM) and induces cell cycle arrest at the G1 phase in MV4-11 cells
Cayman Chemical MyeloperoxIdase MoncLNal n 1ea
Host: Mouse • Species Reactivity: (+) Human, mouse • Application: IF
Revvity Health Sciences Inc HTRF Human & Mouse Total GSK3β Detection Kit, 500 Assay Points
HTRF Human & Mouse Total GSK3β Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress ACVRL1/BMPR2 Dimerization Assay, 2pl Kit. (93-0962E3CP22M)
The PathHunter ACVRL1/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside BMPR2 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 2pl Kit. (93-0968E3CP5M)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXTR Activated GPCR Internalization Assay, 10pl Kit. (93-0715E3CP0L)
The PathHunter OXTR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OXTR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OXTR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OXTR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human BMP-4 ASY
The R-PLEX Human BMP-4 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures the concentration of human Bone Morphogenetic Protein 4 (BMP-4) in serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL
Optimize Technologies EXP Discovery Kit - Contains 1 holder and 3 trap cartridge packs (total of 9 traps). Create your custom kit by choosing a holder (Direct Connect or In-Line) and any 3 of the cartridge configurations.
The patent-pending Hand-Tight EXP™ Trap Column is rated for use at 20,000+ psi (1,400+ bar). This unique design connects directly to any injection valve (with 10-32 threads) or in-line with 1/16” stainless tubing for unparalleled convenience and efficiency. The EXP™ Cartridge System enables chemists to quickly remove detergents or salts which can affect the ionization process in MS work. This trapping technique can concentrate the sample directly on-line, allowing for increased recovery of precious sample material compared to off-line techniques. On-line trapping readily lends itself to automation for high-throughput analysis in UHPLC/MS applications. Free-Turn™ architecture allows the user to change cartridges by hand without breaking fluid connections on the holder inlet/outlet.
Eurofins Discoverx PathHunter Mouse MC4R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse MC4R β-Arrestin Stable Cell Pool Assay (CHO-K1) contains a stable engineered cell pool used to measure mMC4R (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell pool vials, all required culture reagents for maintaining the cells in continuous culture, and detection reagents for running the assay.