General Cell Based Assays
Eurofins Discoverx PathHunter eXpress rDRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0620E2CP2L)
The PathHunter rDRD eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of rDRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rDRD and EA-tagged β-Arrestin-2. Activation of rDRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 24 Reagent, 500. (93-0563R24B)
AssayComplete Cell Plating 24 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress EDNRB CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0593E2CP0L)
The PathHunter EDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of EDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRB and EA-tagged β-Arrestin-2. Activation of EDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 25 Reagent, 100. (93-0563R25A)
AssayComplete Cell Plating 25 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 20 Reagent, 100. (93-0563R20A)
AssayComplete Cell Plating 20 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter Adalimumab Bioassay Kit, 2-Plate. (93-0538B15-00131)
The PathHunter Adalimumab Bioassay Kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, cell plating reagent, control agonist, detection reagents, and assay plates. The ready-to-use cryopreserved cells have been manufactured for single-use to ensure assay reproducibility, and faster implementation from characterization to lot release. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress CCR5 Activated GPCR Internalization Assay, 2-Plate Kit. (93-0653E3CP0M)
The PathHunter CCR5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR85 U2OS β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0409E3CP1L)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR85 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0409E2ACP1M)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2A U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0401E3CP19L)
The PathHunter HTR2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2A and EA-tagged β-Arrestin-2. Activation of HTR2A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR85 U2OS β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0409E3CP1M)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DARC CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0548E2CP1M)
The PathHunter DARC eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of DARC GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DARC and EA-tagged β-Arrestin-2. Activation of DARC stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GALR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0228E2CP0M)
The PathHunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR1 and EA-tagged β-Arrestin-2. Activation of GALR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter BDKRB2 Bioassay Kit, 2-Plate. (93-0189Y2-00113)
The PathHunter BDKRB2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress CCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0195E2CP0L)
The PathHunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR7 and EA-tagged β-Arrestin-2. Activation of CCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.